產(chǎn)品名稱：pEYFP-C1

貨號(hào)	規(guī)格	價(jià)格	訂購(gòu)數(shù)量	是否現(xiàn)貨
ZK2264	1μg(20μl,50ng/μl)	580	- +	有貨

產(chǎn)品介紹

■ 基本信息

啟動(dòng)子:	CMV
復(fù)制子:	pUC ori,f1 ori
終止子:	SV40 poly(A) signal
質(zhì)粒大小:	4731bp
原核抗性:	Kan
真核抗性:	Neo
克隆菌株:	DH5α
培養(yǎng)條件:	37℃，有氧 LB
表達(dá)宿主:	哺乳細(xì)胞
誘導(dǎo)方式:	無須誘導(dǎo)，瞬時(shí)表達(dá)
5'測(cè)序引物:	pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG
3'測(cè)序引物:	Sv40-polyA-R：GAAATTTGTGATGCTATTGC

■ 質(zhì)粒屬性

質(zhì)粒宿主:	哺乳細(xì)胞
質(zhì)粒用途:	蛋白表達(dá)
片段類型:	ORF
片段物種:
原核抗性:	Kan
真核抗性:	Neo
熒光標(biāo)記:

■ 質(zhì)粒簡(jiǎn)介

pEYFP-C1 encodes an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains the four amino acid substitutions previously published as GFP-10C (1): Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and and Thr-203 to Tyr. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 513-nm, the Em of EYFP is 36,500 cm1M1 and the fluorescent quantum yield is 0.63 (1), resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that from EGFP.

■ 質(zhì)粒圖譜

■ 質(zhì)粒序列

質(zhì)粒序列請(qǐng)下載：ZK2264pEYFP-C1哺乳熒光質(zhì)粒.txt