■ 基本信息
啟動子: | CMV |
復制子: | pUC |
質粒分類: | 哺乳細胞，四環(huán)素調控系統(tǒng)載體 |
質粒大小: | 6487bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 四環(huán)素誘導 |
5'測序引物: | 根據(jù)序列設計引物 |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 蛋白表達 |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: | Hyg
|
熒光標記: |
|
■ 質粒簡介
pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tetresponse element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Ψ+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously (2, 3). pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK696pRevTRE哺乳調控質粒.txt
